| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Horne, Neil L (17)
|
ADCPP |
23.78 |
25.22 |
1.44 |
| 2 |
Hotaling, Kaleb B (16)
|
ADCPP |
25.04 |
25.85 |
0.81 |
| 3 |
Ward, Scott T (15)
|
ADUN |
26.48 |
26.29 |
-0.19 |
| 4 |
Verstandig, Kyle J (17)
|
ADCPP |
25.16 |
26.41 |
1.25 |
| 5 |
Homan, Jackson T (15)
|
ADCPP |
25.91 |
26.56 |
0.65 |
| 6 |
Harding, Brian D (17)
|
ADCPP |
26.53 |
26.79 |
0.26 |
| 7 |
Jordan, James V (16)
|
ADCPP |
25.29 |
27.03 |
1.74 |
| 8 |
Salzone, Nicholas V (16)
|
ADCPP |
25.39 |
27.06 |
1.67 |
| 9 |
DeMan, Quinten H (16)
|
ADCPP |
25.60 |
27.19 |
1.59 |
| 10 |
Janeski, John R (14)
|
ADDELM |
27.58 |
27.81 |
0.23 |
| 11 |
Wang, Juneau (15)
|
ADDELM |
27.77 |
27.95 |
0.18 |
| 12 |
Rompicherla, Shrujay S (15)
|
ADCPP |
26.78 |
27.99 |
1.21 |
| 13 |
Ryan, Jack F (17)
|
ADCPP |
28.17 |
28.22 |
0.05 |
| 14 |
Kirkpatrick, Peter R (17)
|
ADCPP |
27.71 |
28.29 |
0.58 |
| 15 |
Burns, Matthew R (14)
|
ADCPP |
27.56 |
28.35 |
0.79 |
| 16 |
Goodspeed, Cameron J (15)
|
ADUN |
28.95 |
28.69 |
-0.26 |
| 17 |
Herbinger, Devin R (15)
|
ADCPP |
28.45 |
29.06 |
0.61 |
| 18 |
Lassetter, Zackary J (15)
|
ADCPP |
28.62 |
29.83 |
1.21 |
| 19 |
Fitzgerald, Colin J (14)
|
ADCPP |
29.58 |
30.39 |
0.81 |
| 20 |
Homan, Jaden (13)
|
ADCPP |
30.97 |
30.50 |
-0.47 |
| 21 |
Constable, Nathan T (16)
|
ADCPP |
29.48 |
31.22 |
1.74 |
| 22 |
Silaika, Xavier R (13)
|
ADCPP |
32.46 |
31.81 |
-0.65 |
| 23 |
Catanzaro, Michael M (16)
|
ADCPP |
32.51 |
31.86 |
-0.65 |
| 24 |
Scott, Eric D (15)
|
ADCPP |
31.28 |
32.41 |
1.13 |
| 25 |
DeVilliers, Etienne M (16)
|
ADCPP |
34.45 |
33.68 |
-0.77 |
| 26 |
Leung, Arthur (10)
|
ADUN |
|
45.54 |
- |
| 27 |
Wang, Alexander (9)
|
ADDELM |
50.55 |
48.45 |
-2.10 |
|
Harnisher, Frank J (17)
|
ADCPP |
|
NS |
- |
|
Cartwright, Brendan J (17)
|
ADDELM |
24.11 |
NS |
- |
|
de Wolf, Samuel R (16)
|
ADCPP |
27.13 |
NS |
- |